Autologe Translokation von Irispigmentepithel auf resorbierbaren Unterlagen als neue therapeutische Methode bei altersbedingter Makuladegeneration (Autologous iris pigment epithelium (IPE) translocation on biodegradable supports as new therapeutic modalit

虹膜色素上皮（IPE）细胞移位术代表了年龄相关性黄斑变性的一种新的治疗方式，其作为手术去除新生血管复合体以替代变性的色素细胞后的辅助治疗。IPE细胞移植物必须具有与原始RPE细胞相同的形态结构才能完全发挥功能，即它们必须形成完整的单层。因此，重要的是，IPE细胞作为已经在单层中的细胞片被移植，或者IPE细胞悬浮液的细胞粘附在老化和损伤的布鲁赫膜存在下通过基底膜材料的共移植而被促进。虽然移植一片细胞在外科手术上是困难的，但这将不仅确保细胞将在单层中，而且它将允许更好的移位和定位在黄斑下，并提供新的基底膜材料。该提案有两个主要部分，将作为四个研究中心的合作努力来开发：一部分涉及天然支持物的开发和将这些片材上的IPE单层移植到视网膜下腔。这一部分导致开发可生物降解膜的程序，这将支持自体IPE的单层生长和功能。第二部分将着重于液体人工可生物降解聚合物凝胶的开发。这些富含细胞外基质蛋白的凝胶将用于培养自体IPE细胞，然后将其注射到视网膜下腔中。这将增强色素上皮对裸露或缺失的布鲁赫膜的再增殖。将进行广泛的体外和体内测试，以确定未来人体应用的最合适的技术。

Iris pigment epithelial (IPE) cell translocation represents a new treatment modality of age related macular degeneration as adjuvant therapy after surgical removal of the neovascular complex to replace the degenerated pigment cells. IPE cell transplants must assume the same morphological structure as the original RPE cells to be fully functional, namely they must form an intact monolayer. Therefore, it is critical that IPE cells are transplanted either as a sheet of cells already in a monolayer or that cell adhesion of IPE cell suspensions is facilitated in the presence of aged and injured Bruch's membrane by co-transplantation of basal membrane material. Although transplantation of a sheet of cells is surgically difficult, this will not only assure that the cells will be in a monolayer, but it will allow better translocation and positioning under the macula and prvides new basal membrane material. This proposal has two main parts which will be developed as the collaborative effort of four study centers: One part involves the development of natural supports and transplantation of IPE monolayers on these sheets into the subretinal space. This part lead to a procedure for developing biodegradable membranes which will support the monolayer growth and function of autologous IPE. The second part will focus on the development of liquid artificial biodegradable polymeric gels. These gels enriched with extracellular matrix proteins will be used to culture autologous IPE cells which will then be injected into the subretinal space. This should enhance repopulation of denuded or missing Bruch's membrane by pigment epithelium. Extensive in vitro and in vivo testing will be performed in order to determine the most sutable technique for future human application.