Mechanisms for the collection of cells by lymphatics

利用形态学和功能方法研究了淋巴管收集细胞的机制。为了从分子基础上对淋巴管进行表征，制备了一种抗小鼠淋巴管的大鼠单克隆抗体（LA1O2）。局部炎症时淋巴管形态和功能的变化：(1)建立了一种番茄电泳血管可视化方法，以区分淋巴管。(2)制备小鼠淋巴内皮细胞特异性单克隆抗体（LA102）。炎症过程中淋巴样细胞与淋巴管的三维关系：(1)在器官移植模型中，炎症细胞在第一次识别阶段使用糖-凝集素结合。(2)用激光共聚焦扫描显微镜研究了它们在微血管中的位置。淋巴内皮细胞和圆形淋巴内皮细胞上富集分子的鉴定(1) LA102抗原是a27- 30kd的稀有蛋白，在淋巴管上表达，而在血管上不表达。(2) LA102抗原与一些细胞外基质和趋化因子的比较研究。LA102分子功能表征：LA102抗原也存在于某些淋巴样细胞表面，提示该分子可能参与细胞与淋巴内皮细胞之间的细胞识别。目前正在研究组织冷冻切片的原位结合分析，以了解LA102抗原的功能方面。这些结果表明LA102。在正常和病理条件下，抗原可能在淋巴管收集淋巴样细胞的机制中发挥重要作用。

The mechanisms for the collection of cells by lymphatic vessels have been investigated using morphological and functional approaches. In order to characterize lymphatics on molecular bases, a rat monoclonal antibody against mouse lymphatics (LA1O2) has been produced.1. Morphological and functional changes in lymphatics during local inflammation:(1) Establishment of a method for visualization of blood vessels by tomato-lection to distiguish from lymphatics. (2)Production of a monoclonal antibody (LA102) specifc to mouse lymphatic endothelial cells.2. 3-dimensional relationship between lymphoid cells and lymphatics during inflammation:(1)In organ transplantation models, inflammatory cells used sugar-lectin binding at the first recognition stage. (2) Their location to microvessels was studied by the confocal laser scan microscopy.3. Identification of molecules rich on and round lymphatic endothelial cells; (1) LA102 antigen is a27-30 kd noble protein expressed on lymphatics but not on blood vessels. (2) LA102 antigen has been under investigation in comparison with some extracellular matrices and chemokines.4. Functional characterization of LA102 molecule:LA102 antigen also exsist on the surface of some lymphoid cells, suggesting that this molecule may be involved in the cellular recognition between the cells and lymphatic endothelial cells. In situ binding assays on tissure cryosections are now under investigation to see the functional aspect of LA102 antigen.These results suggest that LA102. antigen may play an important role in the mechanisms for the collection of lymphoid cells by lymphatic vessels under both normal and pathological conditions.