Detection of lung adenocareinoma cells in peripheral blood by RT-PCR analysis for mRNA of surfactant protein

1期肺癌患者的5年生存率仅为60- 70%。肺癌的预后通常会因恶性播散而受损。采用RT-PCR方法检测外周血中SP-A、SP-C和CC 10的mRNA表达。19例健康志愿者外周血中SP-A、SP-C和CC 10 mRNA均为阴性。99例非小细胞肺癌中33例（33.3%）SP-AmRNA阳性。SP-CmRNA（14%）和CC 10 mRNA（8%）的阳性率较低。Ⅰ期患者SP-A mRNA阳性表达率为26%，显著高于SP-C mRNA（8%）和CC-10 mRNA（3%）。SP-AmRNA表达阳性标本经手术治疗后全部消失，RT-PCR检测SP-AmRNA可用于外周血中肺腺癌隐匿性转移的检测。

The 5 years survival of lung cancer patients with stage.1 is only 60-70%. The prognosis of lung cancer is usually impaired with melastalic dissemination. To detect circulating lung cancer eelIs in peripheral blood, we performed RT-PCR analysis for mRNA of SP-A, SP-C and CC10.The 19 samples from healthy volunteers were all negative for SP-A, SP-C, CC10 mRNA.expression. SP-A mRNA was detected in 33 patients (33.3%) with 99 nonsmall cell lung cancer. The positive rale of SP-CmRNA(14 %) and CC10mRNA(8 %) were low. The positivity of expression for SP-A mRNA in the patients with stage 1 was 26 %, and the positive rate of SPA mRNA was higher than SP-C mRNA (8 %) and CC-10mRNA(3 %) significantly. The positive samples of SP-A mRNA expression disappeared with all cases after a curative surgical operation.RT-PCR analysis for SP-A mRNA is clinically useful for the detection occult metastatic lung adenocarcinoma cells in periferal blood.