An epidemiological study of cariogenic and periodontopathic bacteria in children

变异链球菌是人类龋病的始动菌已被公认。人类最常见的菌种是变异链球菌和远缘链球菌。成人牙周病与牙龈卟啉单胞菌和中间普氏菌的存在有关。此外，局限性青少年牙周炎是由伴放线放线杆菌。本研究旨在探讨不同口腔健康状况儿童龈上菌斑中致龋菌和牙周致病菌的存在情况。齿数（单位：在收集龈上菌斑之前，测定每个受试者的d-需要填充的龋齿; f-填充的龋齿和DMF：D-龋齿; M-缺失的牙齿; F-填充的牙齿）、PMA指数（P-乳头牙龈炎; M-边缘牙龈炎; A-附着牙龈炎）、OHI（口腔卫生指数）和口腔恶臭。致龋菌（链球菌）的检测 ...更多信息 sobrinus）在龈上菌斑中的表达。患龋儿童与非患龋儿童在牙数、口臭等临床指标上有显著性差异。S.患龋儿童的远缘龋显著高于无龋儿童。此外，观察龋病儿童口腔异味与碎屑指数的关系。牙周致病菌（牙龈卟啉单胞菌、中间卟啉单胞菌、A.使用免疫印迹bot测定法，用对每个微高潮特异的单克隆抗体检测龈上菌斑中的放线菌属（actinomycetemcomitans）。在两个受试者组（有龋和无龋儿童）中，三种牙周病细菌的频率没有差异。牙龈卟啉单胞菌阳性者，而中间卟啉单胞菌、A.伴放线菌对应物与口腔恶臭相关。口腔恶臭也与OHI的一个组成部分碎片指数相关。具有较高OHI的组显示出较高的牙周病细菌患病率。对受试者的三种牙周致病菌，牙龈卟啉单胞菌，中间卟啉单胞菌和A。伴放线菌阳性菌斑与年龄无关。儿童的龈上菌斑可携带牙龈卟啉单胞菌、中间卟啉单胞菌和A.伴放线菌少

It has been generally accepted that mutants streptococci is an initiative bacterium in human dental caries. The most common species encountered in human are Streptococcus mutants and Streptococcus sobrinus. Adult periodontal disease is associated with the presence of Porphyromonas gingivalis and Prevotella intermedia. Also, localized juvenile periodontitis is caused by Actinobacillus actinomycetemcomitans. The present study was to determine the presence cariogenic and periodontopathic bacteria in the supragingival plaques of children with different oral health conditions. Tooth number (in df: d-decayed deciduous teeth indicated for filling; f-filled deciduous teeth and DMF: D-Decayed teeth; M-Missing teeth; F-Filled teeth), PMA index (P-papillary gingivitis; M-marginal gingivitis; A-attached gingivitis), OHI (oral hygiene index) and oral malodor of each subject were determined prior to the collection of the supragingival plaques. The detection of the cariogenic bacteria (Streptococcus … More sobrinus) in the supragingival plaques was carried out using ELISA with anti-serum. A significant difference between children with caries and without caries was observed in df tooth number and also in oral malodor in clinical parameters. S. sobrinus in children with caries was significantly higher than that in without caries. In addition, the relationship between the oral malodor in children with caries and the debris index was observed. Periodontopathic bacteria (P. gingivalis, P. intermedia, A. actinomycetemcomitans) in the supragingival plaques were detected using an immunoslot bot assay with monoclonal antibodies specific for each micro-orgasm. The frequencies of the three periodontopathic bacteria in the two subject groups (children with and without caries) tested were not different from each other. P. gingivalis positive subjects, but not P. intermedia, A. actinomycetemcomitans counterparts were correlated to oral malodor. Oral malodor was also correlated to debris index, a component of OHI. The group with a higher OHI showed a higher prevalence of periodontopathic bacteria. For the three periodontopathic bacteria in the subjects tested, P. gingivalis, P. intermedia and A. actinomycetemcomitans positive plaques were not age-related. The supragingival plaques in children can harbor periodontopathic bacteria such as P. gingivalis, P. intermedia and A. actinomycetemcomitans. Less