Elucidation of activation mechanism for immune response in hyperthermia using MCL and application to cancer therapy

1.磁性阳离子脂质体（MCL）对多种肿瘤动物模型（兔、仓鼠、大鼠和小鼠）的细胞热疗效果显著。利用磁性脂质体进行选择性热疗可诱导淋巴结免疫应答。免疫细胞（NK细胞、T细胞和树突状细胞）被激活。利用amlantibody偶联磁脂质体（AML）对肿瘤细胞和细胞内热疗的靶向能力进行了研究。抗肾细胞癌单克隆抗体与磁性脂质体结合。静脉注射急性髓细胞白血病时，急性髓细胞白血病仅积累肾细胞癌，细胞内热疗非常有效。热疗对人类前列腺癌和遗传性黑色素瘤小鼠模型的影响热能杀死任何细胞。这不仅适用于小鼠或大鼠癌细胞，还适用于小鼠模型中的人类前列腺癌和遗传性黑色素瘤。反复热疗比单次热疗更有效。热休克免疫应答的激活机制应用热疗，产生大量热休克蛋白。热休克蛋白70是肿瘤肽的伴侣蛋白，可诱导对肿瘤的免疫反应。

1.Hyperthermia using MCL for various carcinoma cellsIntracellular hyperthermia using magnetite cationic liposomes (MCL) was very effective for various carcinoma animal models (rabbit, hamster, rat and mouse).2.Immune induction in lymph node using MCL-hyperthermia systemSelective hyperthermia using magnetoliposomes was found to induce immune responces in lymph node. Immune cells (NK cells, T cells and dendritic cells) were activated.3.Targeting ability in tumor cells and intracellular hyperthermia using AMLAntibody-conjugated magnetoliposomes (AML) were developed. The monoclonal antibody to renal cell carcinoma was bound to the magnetoliposomes. This AML accumulated only renal cell carcinoma when AML was injected in vein, and intracellular hyperthermia was very effective.4.Hyperthermia for human prostate cancer and hereditary melanoma in a mouse modelHeat can kill any cells. This was checked not only for mouse or rat carcinoma cells, but also for human prostate cancer and hereditary melanoma in a mouse model. Repeated hyperthermia was more effective compared with a single hyperthermia treatment.5.Activation mechanisms for immune response in hyperthermiaBy applying hyperthermia treatment, a lot of heat shock proteins were produced. Heat shock protein 70 chaperons tumor peptides, which induces immune response to the tumor.