Monitoring Strategies and Tools to address knowledge gaps on aquatic Fungal biodiversity

水生真菌（AF）控制着关键的生态系统过程，包括支持、供应、支持和调节服务。AF负责养分循环、污染物分解和控制微藻华。然而，它们没有被纳入任何常规和大规模的生物多样性监测计划。MoSTFun是一项泛欧倡议，汇集了8个欧洲合作伙伴、世界自然保护联盟物种生存委员会和GEOBON专家的互补专业知识，以发展对大多数欧洲水生生态系统中的AF及其服务的监测，从淡水到咸淡水和沿海，跨越很大的纬度范围。MoSTFun的策略是以经济高效的方式利用现有资源：冷冻室、档案和数据存储库是未开发的样本和数据来源（THEME3）。通过伙伴关系和合作，MoSTFun前所未有地获得了来自生物多样性监测计划和长期研究计划的数据和样本。这将使MoSTFun能够填补整个欧洲AF多样性的知识空白（THEME2）。首先，我们将优化和应用最先进的组学技术和采样策略（WP4），从现有的DNA档案和案例研究中获得关于AF多样性和驱动因素的标准化数据，这些数据来自冰川和海岸河口环境等研究不足的地区（WP1,2）。我们还将挖掘公共生物多样性和遗传数据库，以了解现有资源的潜力，从不同的数据源揭示AF多样性（WP1）。将新生成和挖掘的数据与从可用数据库（也包括环境变量）、地球观测（EO、卫星）和GIS数据以及利益相关者获得的知识相结合和集成，我们将开发数据集成管道，以建立AF生物多样性和驱动因素跨空间和时间模式的模型，并最终输入一套新的基本生物多样性变量（WP3）。这将揭示真菌在水生环境中提供的生物多样性和关键服务的全球理解的有用性和潜力。我们将进一步测试我们的优化方法（WP4），用于监测新出现的地球健康问题，即抗真菌抗性基因在环境中的传播（WP4）。在新的知识到行动中心（WP5）的支持下，在利益相关者的参与下，我们还将评估和制定欧洲水域AF生物多样性监测（THEME1）的最有效战略和工具。MoSTFun联盟包括跨学科专家，促进互补技能和专业知识的交流，为早期职业研究人员的培训和流动做出贡献。因此，MoSTFun将为将非洲农业生物多样性纳入欧洲常规监测计划和战略创造临界质量，以及社会和政策影响，并具有长期的跨国效益。

Aquatic fungi (AF) govern critical ecosystem processes involved in supporting, provisioning, supporting, and regulation services. AF are responsible for nutrient cycling, breakdown of pollutants, and control of microalgal blooms. However, they are not included in any routine and large-scale biodiversity monitoring programs. MoSTFun is a pan-European initiative that brings together the complementary expertises of 8 European partners, the IUCN Species Survival Commission, and GEOBON specialists to develop the monitoring of AF and their services in most European aquatic ecosystems, from freshwater to brackish and coastal, across a large latitudinal range. MoSTFun’s strategy is to capitalise on existing resources with a cost-efficient approach: freezers, archives, and data-repositories are untapped sources of samples and data (THEME3). With its partnerships and collaborations, MoSTFun has unprecedented access to data and samples from biodiversity monitoring programmes and long-term research initiatives. This will enable MoSTFun to fill knowledge-gaps on AF diversity throughout Europe (THEME2). First, we will optimise and apply the most advanced and cutting-edge -Omics technologies and sampling strategies (WP4) to produce standardised data on AF diversity and drivers from existing DNA archives and case studies in understudied areas like glaciers and coastal-estuarine environments (WP1,2). We will also mine public biodiversity and genetic databases to understand the potential of the resources already available to unravel AF diversity from different data sources (WP1). Combining and integrating newly generated and mined data with data obtained from available data-repositories (also including environmental variables), earth observation (EO, satellite) and GIS data, and knowledge gain from stakeholders, we will develop pipelines for data integration needed to model patterns of AF biodiversity and drivers across space and time, and ultimately feed into novel set of Essential Biodiversity Variables (WP3). This will reveal the usefulness and the potential for global understanding of biodiversity and key services provided by fungi in aquatic environments. We will further test our optimised methods (WP4) for use in monitoring of an emerging planetary health issue, i.e. the spread of antifungal resistance genes in the environment (WP4). With stakeholder engagement (all WPs) supported by a novel Knowledge-to-Action Hub (WP5), we will also assess and develop the most effective strategies and tools for AF biodiversity monitoring in waters across Europe (THEME1). The MoSTFun consortium includes interdisciplinary experts, fostering the exchange of complementary skills and expertise, contributing to the training and mobility of early career researchers. MoSTFun will thus create critical mass, as well as societal and policy impact for the inclusion of AF biodiversity in European routine monitoring programs and strategies, with long-lasting transnational benefits.