Establishment of evidence based antibacterial chemotherapy

研制了一种新型全自动流式细胞仪尿液细菌分析仪UBA。我们评估了UBA测量的线性、结果的可重复性、携带率以及测量结果与尿培养结果的相关性。我们还评估了其筛选尿液样本中显著细菌尿的能力。UBA表现出良好的线性和较小的结转率。UBA的结果具有很高的可重复性，并且在运行间精确分析中，UBA结果的变异系数小于尿培养结果。对大学附属医院门诊患者尿液标本273份进行了检测。将UBA的结果与尿培养的结果进行比较。UBA检出显著细菌的敏感性为96.6%，特异性为79.9%，阳性预测值为57.0%，阴性预测值为98.8%，假阳性率为15.8%，假阴性率为0.7%，准确率为83.5%。这些结果与先前报道的筛选程序所获得的结果相当或更好。UBA可在90秒内自动准确计数细菌细胞，可用于重要菌尿的筛选。此外，UBA可用于开发一种新的装置来确定尿路感染细菌的敏感性。

A new automated flow cytometry based urine bacteria analyzer, UBA, was developed. We assessed the UBA for linearity of measurement, reproducibility of results, carryover rate, and correlation of the measured results with those determined by urine culture. We also evaluated for its ability to screen urine samples for significant bacteriuria.The UBA showed excellent linearity and minor carryover rate. Results from the UBA were highly reproducible, and in between-run precision assays, the coefficients of variation for UBA results were smaller than those by the urine culture.Two hundred and seventy-three urine specimens from patients attending outpatient clinic of university based hospital were examined. The results from the UBA were compared with those from urine culture.The UBA detected significant bacteria with sensitivity 96.6%, specificity 79.9%, positive predictive value 57.0%, negative predictive value 98.8%, false positive rate 15.8%, false negative rate 0.7%, and accuracy 83.5%. These results were comparable to or better than those obtained with previously reported screening procedures.The UBA can perform accurate enumeration of bacterial cells automatically in 90 seconds, and can be used for the screening of significant bacteriuria. Moreover, the UBA can be used to develop a new device to determine the susceptibilities of bacteria in urinary tract infections.