The mRNA expression of neuropeptide-receptor on the cells around the alveolar bone tinder the stressful condition in rats.

牙周病是由牙周炎相关微生物感染引起的炎症性疾病。我们以前曾报道过束缚应力引起大鼠上颌右第二磨牙（M2）根分叉区牙槽骨吸收。另一方面，据报道，情绪和/或心理压力与磨牙症之间存在正相关。同时也有报道磨牙症可能会影响牙周病的进展。然而，我们还没有阐明咬合对这种骨吸收的影响。因此，我们证实，即使在没有咬合力的情况下，大鼠的牙槽骨吸收也在进行。结果提示，约束应力可引起牙槽骨严重丢失，并伴有牙周炎症，而咬合力对牙槽骨丢失无明显影响，而串珠状神经元数目的增加则可引起牙槽骨严重丢失 ...更多信息 P物质（Substance P，SP）是一种具有神经递质或神经调质作用的神经肽。SP阳性神经纤维广泛分布于脑、肠、免疫系统、骨和牙龈组织中，SP受体（SPR）也分布于多种细胞中。本研究采用原位杂交方法，观察了束缚应激条件下大鼠根分叉区牙槽嵴周围细胞SPR mRNA的表达，结果表明：应激大鼠M2分叉区牙槽骨周围SPR阳性细胞数明显增多，TRAP阳性细胞数明显增多，提示束缚应激可能解除。外周神经末梢的SP可诱导牙槽骨周围细胞表达SPR mRNA，并与牙槽骨丢失加重有关，提示束缚应激可调节牙周炎症的进展，SP是应激与牙周病相互作用的关键物质之一。少

Periodontal disease is an inflammatory disease caused by the infection of periodontitis related microorganisms. The progression is modified by the host immune response.We have previously reported that the restraint stress induced the remarkable alveolar bone resorption in the furcation area of the maxillary right second molar (M2) of rat. On the other hand, it is reported that there exist positive link between emotional and/or psychological stress and bruxism. And it is also reported bruxism may have effects on the progression of periodontal disease. However, we have not been clarified the effect of occlusion on this bone resorption. Therefore, we confirmed that the alveolar bone resorption was progressed even in the condition without occlusal force in rats. These results suggest that the restraint stress may induce the severe alveolar bone loss together with periodontal inflammation, and such bone loss may not be influenced by occlusal force.On the other hand, an increase in beaded ne … More rve terminals occurred around the vessels in the furcation area when the rats were exposed to a restraint stress.Substance P (SP) is one of neuropeptides that work as neurotransmitters or neuromodulators. The SP-positive nerve fibers are distributed in the brain, intestine, immune system, bone and gingival tissue, and SP receptors (SPR) are also distributed in many kinds of cells. These distribution suggests that SP may directly modulate immune response and bone netabolism through SPR.In this study, we investigated the expression of the messenger RNA (mRNA) of SPR in the cells around the alveolar e of the furcation area under restraint-stressful condition in rats by using in situ hybridization method.The results of this study were as follows: The number of SPR-positive cells around the alveolar bone in the furcation area of M2 in stress rat were significantly increased and TRAP-positive cells around the alveolar bone in the furcation area of M2 had significantly increased.These results suggests that the restraint stress may release. SP from the endings of the peripheral nerve, induce the expression of SPR mRNA in the cells around the alveolar bone and be related to the enhancement of alveolar bone loss.We conclude that the restraint stress modulates the progression of periodontal inflammation and SP is one of the key substances for interaciting the association between stress and periodontal disease. Less