Morphological and functional characterization of a specific marker for lymphatic vessels.

利用鼠单抗LA102对小鼠淋巴管的特异性标志物进行了形态和功能的鉴定。1.LA102抗体的特异性及其抗原的组织分布：(1)LA102能识别小鼠淋巴管，但不能识别血管。(2)LA102识别的抗原定位于淋巴管内皮细胞腔内病细胞膜和基底病细胞膜，也高度浓缩在胞饮细胞膜和运输囊泡膜上。(3)从良性淋巴管瘤中分离出LA102阳性内皮细胞，建立细胞系。2.LA102抗原的免疫生物学特性：(1)LA102抗体亚型为大鼠κ。(2)LA102抗原是一种糖蛋白，分子大小为25-27 kDa。(3)LA102抗原主要表达于T淋巴细胞和脑组织。3.LA102抗原的分子和遗传学特征：(1)LC-MS分析表明，LA102抗原具有与CD90分子相似的氨基酸序列。(2)LA102抗原与CD90及其他相关分子(如细胞黏附分子、趋化因子)的遗传学分析正在进行中。4.LA102抗原的功能特性：(1)建立了多种细胞从腹膜腔内迁移的实验模型。腹膜和大网膜是腹膜外淋巴的主要来源。(2)在炎症模型中，LA102抗体似乎能抑制细胞与多种组织的结合，如肝、胸腺、淋巴结。这些结果提示，在正常和病理条件下，LA102可能在淋巴管内淋巴样细胞的迁移和某些转运机制中发挥重要作用。

A specific marker for mouse lymphatic vessels has been characterized morphologically and functionally using a rat monoclonal antibody, LA102. 1.Specificity of LA102 antibody and tissue distribution of its antigen : (1)LA102 recognized mouse lymphatic vessels, but not blood vessels. (2)The antigen recognized by LA102 was localized on both luminal-and basal-sick cell membrane of lymphatic endothelium and was also highly condensed on the pinocytic or transport vesicle membrane. (3)LA102 positive endothelial cells were isolated from a benign lymphangioma and established as a cell line. 2.Immunobiological characterization of the LA102 antigen : (1)The isotype of LA102 antibody was rat IgG2b,κ. (2)The LA102 antigen was a glycoprotein with a molecular size of 25-27kDa. (3)LA102 antigen was also expressed on mainly T lymphocytes and cerebral tissues. 3.Molecular and genetical characterization of LA102 antigen : (1)LC-MASS analyzes revealed that LA102 antigen has a similar amino acid sequence to CD90 molecule. (2)Genetical analyzes of LA102 antigen have been underway comparing with CD90 and some other related molecules (such as cell adhesion molecules and chemokines). 4.Functional characterization of LA102 antigen : (1)Several experimental models for cell migration from the peritoneal cavity have been established. The diaphragm and omentum were the main lymphatic routes from the peritoneal cavity. (2)In an inflammatory model, LA102 antibody seemed to inhibit cell binding to various tissues, such as the liver, thymus, lymph nodes. These results suggest that LA102 might play an important role in the lymphoid cell migration and some transporting mechanisms through lymphatics under both normal and pathological conditions.