Studies on emerging diseases derived from Primates and Chiroptera

1.埃博拉病毒和B病毒的研究建立了表达埃博拉病毒核蛋白的HeLa细胞免疫荧光技术和表达埃博拉病毒抗原的杆状病毒ELISA方法，用于灵长类动物血清中埃博拉病毒抗体的检测。利用表达的B病毒gD蛋白建立了荧光ELISA。该方法可检测出灵长类动物抗B病毒的特异性抗体，与人类疱疹病毒无交叉反应.蝙蝠免疫系统的研究制备抗蝙蝠IgG兔血清，建立蝙蝠血清中病毒抗体的常规ELISA检测方法。该抗蝙蝠IgG兔血清与测试的Mega和Microbats的IgG反应。此外，我们还测定了蝙蝠CD 4、IgFcRn、IFN-α亚型1和INF-β编码区的核苷酸序列.蝙蝠病毒的血清学调查建立了标记抗生物素蛋白-生物素酶联免疫吸附试验（ELISA）检测蝙蝠中横濑病毒抗体的方法。为了检查这种ELISA的可用性，在菲律宾和马来西亚收集的蝙蝠血清样本进行。来自菲律宾的2.7%和来自马来西亚的19%的样本具有可检测的抗体。我们还进行了实验感染横濑病毒的食果蝙蝠。结果显示，该病毒在蝙蝠中的复制能力很差，这表明蝙蝠似乎不是横濑病毒的扩增宿主。

1. Research on Eboravirus and B virusImmunofluorescent technique using HeLa cells expressing Eboravirus nucleocapsid protein and, ELISA method with baculovirus expressed Eboravirus antigen were established to detect antibody against the virus from primates. We also established fluorometric ELISA using expressed B virus gD protein. This method could detect specific antibody against B virus from primates with no cross reactivity to human herpesviruses.2. Research on bat immune systemAnti-bat IgG rabbit serum was produced to establish conventional ELISA method for the detection of viral antibody from bat sera. This anti-bat IgG rabbit serum reacted with IgG of Mega and Microbats tested. Moreover, we determined the nucleotide sequences of the bat CD4, IgFcRn, IFN-α subtype 1, and INF-β coding region.3. Serological survey of bat virusesThe labeled avidin-biotin ELISA system was established to detect antibody against Yokose virus from bats. To examine the availability of this ELISA, bat serum samples collected in the Philippines and Malaysia was conducted. 2.7% of the samples from the Philippines and 19% from Malaysia had detectable antibodies. We also conducted experimental infection of Yokose virus to frugivorous bats. The results showed poor replication of the virus in bats, suggesting that bats do not seem to serve as an amplifying host for Yokose virus.