Pathogenic proteomics on the expression of pathogenesis of the new lethal neuropathogenic herpesvirus

疱疹病毒具有很强的宿主特异性。在它们急性感染后，它们会潜伏在宿主体内感染。然而，疱疹病毒意外地越过物种屏障，在外来宿主中引起致命的严重神经系统疾病我们从表现脑炎的汤姆森瞪羚中分离出一种新的疱疹病毒，瞪羚疱疹病毒1(GHV-1)。由于GHV-1与马疱疹病毒1(EHV-1)的亲缘关系，现在将其命名为马疱疹病毒9(EHV-9)。已知EHV-9在瞪羚、山羊、狗、猫、老鼠和仓鼠中会引起致命性感染，在马、牛和猪中也会引起隐性感染。毒力程度的差异现在已经很明显了。我们计划通过蛋白质组的方法研究与EHV-9致病和毒力相关的基因和蛋白。在实验的原因中，我们发现由于蛋白质组学方法的灵敏度较低，因此检测EHV-9致病性差异的蛋白质组学方法是有限的。因此，我们增加了使用EHV-1进行比较分析的另一种方法。通过对EHV-9和EHV-1的分析，揭示了EHV-9和EHV-1的致病性和毒力的基因和蛋白。

Herpesviruses have a strong host specificity. After their acute infection, they latently infect in the host. However, the herpesvirus accidentally jumps the species barrier to cause the lethal severe neurological disease in the alien host We have isolated a new herpesvirus, Gazelle Herpesvirus 1 (GHV-1), from Thomson's gazelles showing encephalitis. GHV-1 is now assigned as Equine herpesvirus 9 (EHV-9) because of the phylogenic relatedness to the equine herpesvirus 1 (EHV-1). EHV-9 is known to cause lethal infection in gazelles, goats, dogs, cats, mice and hamsters as welll as inapparent infection in horses, cattle and pigs. The differences of the degree of virulence is now clear. We planned to investigate genes and proteins involving the pathogenesis and virulence of EHV-9 by protemics approach. During the cause of experiments, we found that the proteomics had a limit to detect such differences of EHV-9 pathogenicity because of the low sensitivity of the proteomics assay. Therefore we have added another approach of comparative analyses by using EHV-1. Analyzing EHV-9 and EHV-1, we revealed the genes and proteins of EHV-9 and EHV-1 in their pathogenicity and virulence in this project.