Study on the reproductive isolation and speciation of the Fejervarya limnocharis complex from Asia

稻蛙（Fejervarya limnocharis）是亚洲分布最广的蛙类之一。虽然物种内部的形态差异很小，但该物种的种群之间和种群内部的交配叫声和遗传特征存在显着差异。因此，Fejervarya limnocharis（以下简称F. limnocharis复合体）被认为是许多不同物种的复合体。本研究的目的是确定（1）该复合体的学名为Fejervarya limnocharis，（2）该复合体与整个亚洲有多大的遗传差异，以及它具有什么样的地理分布模式，（3）该复合体的遗传差异与生殖隔离的发生和机制有什么样的相关性，（4）将该种复合体从蛙属中分离出来并归入蛙属是否有效。在这项研究中，首先，样品的泽漆复杂， ...更多信息 X是从亚洲广泛的分布区收集的。使用它们，进行了广泛的杂交实验。在此基础上，初步探讨了生殖隔离的发生、机制及其程度。从生物种的概念出发，直接论证了该复合体的物种多样性。物种多样性在繁殖隔离机制上只有在两栖生物研究所才能得到独特的体现，因为我所拥有一套完整的繁殖设备和杂交系统。此外，在这项研究中，DNA和等位酶分析和形态测量进行估计复杂内的遗传分化程度。比较这些。根据上述杂交实验的结果，从多个角度证明了复合体的多样性，并为尚未明确定义的复合体的分类建立了更客观和可靠的系统。本研究结果可为多种动物种复合体的多样性和分类研究提供重要参考。少

The rice frog (Fejervarya limnocharis) is one of the most widely distributed species among Asian frogs. While there is little morphological difference within the species, there are significant differences in mating calls and genetic traits between and within populations of this species. Therefore Fejervarya limnocharis (henceforth referred to as the F. limnocharis complex) is considered to be a composite of many different species. The purpose of this study is to determine (1) which species is exactly referred to as the scientific name of Fejervarya limnocharis, (2) how much genetic divergence occurs in this complex from the entire Asia, and what geographical distribution patterns it has, (3) what correlation the genetic divergence of this complex has with the occurrence and mechanism of reproductive isolation, and (4) whether it is valid to separate this species complex from the genus Rana and include it in the genus Fejervarya. In this study, first, samples of the E limnocharis comple … More x were collected from a wide range of distribution areas in Asia. Using them, extensive hybridization experiments were performed. Based on the results, the occurrence and mechanism of reproductive isolation as well as its levels were identified. Consequently, the species diversity of the complex was directly demonstrated based on the biological species concept. The diversity of the species could be uniquely demonstrated in terms of reproductive isolation mechanism only in the Institute for Amphibian Biology, because our Institute has a complete set of breeding equipment and hybridization systems. Also in this study, DNA and allozyme analyses and morphometric measurements were performed to estimate the degree of genetic divergence within the complex. Comparing these. estimations with the results of the above hybridization experiments, the diversity of the complex was demonstrated from multiple viewpoints and a more objective and reliable system was established for classification of the complex that had not been clearly defined. The present results can greatly contribute to the studies on diversity and classification in the species complex of many animals. Less