Clinical applicdtion of SNP analysis in liver diseases

我们探讨了SNP分析在NASH和单纯性脂肪变性的差异、2)重型肝炎、3)干扰素治疗丙型肝炎的临床意义。1)NASH患者-1031C和-863A位基因多态频率显著高于单纯性脂肪变性患者。影响肿瘤坏死因子产生和胰岛素抵抗的肿瘤坏死因子基因多态性可能与NAFLD的进展有关。2)IL-10单倍型和肿瘤坏死因子-β基因B2/B2携带率均显著高于对照组。IL-10、肿瘤坏死因子-β等细胞因子基因的多态性可能与FH的发病有关。3)在肿瘤坏死因子-β基因多态性中，无应答者的B2/B2频率显著高于瞬时应答者和持续应答者。血清分型、肝纤维化和肿瘤坏死因子-β基因多态性可能与联合治疗无效有关。

We investigated the clinical meaning of SNP analysis in 1) difference between NASH and simple steatosis, 2) fulminant hepatitis, 3) effect,of IFN therapy in hepatitis C virus.1) The carrier frequencies of polymorphisms at positions -1031 C and -863 A were significantly higher in patients with NASH than in those with simple steatosis. TNF polymorphisms, which influence TNF production and insulin resistance, might be associated with the progression of NAFLD. 2) The carrier rate with both IL-10 haplotype and TNF-β gene B2/B2 was significantly higher than control. Variations of cytokine polymorphisms including IL-10 and TNF-β genes may be attributable to the pathogenesis of FH. 3) In TNF-β gene polymorphism, B2/B2 frequency was significantly increased in non-responder, compared with transient responder and sustained responder. Serotype, liver fibrosis and TNF-β gene polymorphism might be associated with non-responders to the combined therapy.