Improving clostridial toxoid production through molecular fermentation maps

该项目旨在通过生成详细的发酵分子图谱来改进疫苗生产，这些图谱将用于设计更优的发酵工艺，并降低成本。类毒素疫苗是畜牧业常用的预防致病性梭状芽胞杆菌引起的动物疾病的疫苗，采用分批发酵工艺生产。在过去的50年里，这些流程经历了有限的优化。低滴度和频繁的批次失败极大地影响了资本的使用，并带来了巨大的成本。此外，目前的优化方法受到使用昂贵和噪声的终点分析的限制。该项目旨在使用高通量化学(多组学)来克服这些限制。

This project aims to improve vaccine production by generating detailed molecular maps of fermentation which will be used to design superior fermentation processes with reduced cost. Toxoid vaccines, used routinely in the livestock industry to prevent animal-disease caused by pathogenic Clostridia, are produced using batch fermentation processes. These processes have undergone limited optimisation over the past five decades. Low titres and frequent batch failures greatly affect capital use and represent a significant cost. In addition, current optimisation approaches are limited by the use of expensive and noisy endpoint assays. This project aims to use high-throughput chemistry (multi-omics) that overcome these limitations.