Analysis of the heat stress transcription factors network of Arabidopsis thaliana

植物逆境反应系统的复杂性与调控蛋白的异常多样性有关。对拟南芥基因组的分析发现，存在21个编码热应激转录因子（Hsfs）的ORFs。该项目旨在比较分析这些Hsfs的表达模式、激活子功能、细胞内定位以及最重要的是它们与其他Hsfs（异齐聚化）或其他调节蛋白（共调节蛋白）的相互作用。一个中心方面将是拟南芥hs基因的复杂启动子结构的特征，以及hsf组合在有效激活这些基因中的作用（hsf之间的协同作用）。前三年的研究结果将为进一步研究Hsf网络及其与植物一般胁迫信号通路的相互作用奠定基础。

The complexity of the plant stress response systems correlates with an unusual multiplicity of regulatory proteins. Analysis of the Arabidopsis genome revealed the existence of 21 ORFs encoding heat stress transcription factors (Hsfs). The project aims at the comparative analysis of these Hsfs with respect to their expression patterns, activator functions, intracellular localization and above all their interaction with other Hsfs (heterooligomerization) or other regulatory proteins (coregulators). A central aspect will be the characterization of the complex promoter architecture of the Arabidopsis hs genes and the role of hsf combinations for efficient activation of these genes (synergism between Hsfs). The results from the first three years period will serve as a basis for more advanced investigations of the Hsf network and its cross talks with the general stress signalling pathways of plants.