Strain typing of Candida alhiranstargeting to repeat sequences in each chromosome

白色念珠菌是健康人和免疫功能低下者念珠菌病的最主要致病酵母菌。念珠菌病的监测和预防，应重点区分念珠菌。菌株水平的白色念珠菌。本研究对25 SrDNA和ALT重复序列进行了PCR检测。此外，微卫星重复序列片段分析也适用于进一步的基因型研究。菌株水平的白色念珠菌。179株临床分离株根据25 S rDNA分为A型92株、B型37株和C型50株，并通过针对ALT重复序列的PCR扩增将每种基因型进一步分为3、4、3/4、2/3/4和3/4/5 5型。3 C型。用CDC 3、HIS 3、CAI和CA Ⅲ进行微卫星片段分析，结果表明，25 SINT、P-Ⅱ和AS-Ⅰ的电泳图谱基本一致，可区分不同基因型的菌株。相反，使用25 SINT、P-II和AS-I的PCR区分在片段分析中显示相同分数的菌株。这些结果表明，三种不同系统的组合是有用的C基因分型。菌株水平的白色念珠菌（DP=99.99）。对117株C.在巴西、日本和泰国分离的白色念珠菌表明，每个国家存在特异性基因型

Candida albicans is the most etiologic yeast species of candidiasis in both healthy and immunocompromized individuals. For surveillance and prevention of candidiasis, it is important to discriminate C. albicans at the strain level. PCRs targenting 25S rDNA and ALT repeat element have evaluated in this study. Furthermore, fragment analysis of microsatellite repeats was adapted to further genotype study of C. albicans at the strain level. One hundred-seventy nine clinical isolates were grouped into genotypes A(92 isolates), B(37 isolates)and C(50 isolates)on the basis of their 25S rDNA, and each was further classified into 5 types(types 3, 4,3/4,2/3/4 and 3/4/5)by PCR amplification targeting ALT repeats. Type 3 C. albicans constituted the majority of isolates in any genotypes(66.3% for genotype A, 78.3% for genotype B and 72.0% for genotype C).The fragment analysis of microsatellites using CDC3, HIS3, CAI and CAIII allowed to discriminate the strains, which showed the same electophoretic patterns in the PCRs using 25SINT, P-II and AS-I. Conversely, the PCRs using 25SINT, P-II and AS-I discriminated the strain that showed identical scores in the fragment analysis. These results indicate that a combination of three different systems is useful for genotyping of C. albicans at the strain level(DP=99.99). Furthermore, the fragment analysis using 117 strains of C. albicans isolated in Brazil, Japan and Thailand suggested the presence of genotypes specific for each country