Analysis of Uropathogenic-specific protein and development of the mucosal vaccine by using of USP

用结晶紫结合试验检测了194株急性膀胱炎、76株肾盂肾炎和107株前列腺炎分离株的生物被膜形成能力。前列腺炎分离株的OD值显著高于膀胱炎和肾盂肾炎分离株。同样，经常从前列腺炎中分离出来的O4和O22血清型菌株的OD值明显高于其他菌株。此外，对21株前列腺炎分离株进行卷曲菌毛表达检测时，12株OD值高的菌株中有8株表达卷曲菌毛，而9株低OD值的菌株中只有3株表达卷曲菌毛。这些结果表明急性细菌性前列腺炎和生物被膜形成之间存在关联(Kanamaru S.等人，Int.J·乌罗尔。来自非复杂性尿路感染的427株大肠埃希菌(膀胱炎194株，肾盂肾炎76株，前列腺炎107株)和50株粪便分离株进行了系统发育分型和PAI-USP亚型的…分析以及毒力因子(VFS)和O型血清群的流行情况。在膀胱炎、肾盂肾炎和前列腺炎中，B2群和USP阳性株同样占优势。此外，每种PAI-USP亚型都与UPEC的几个VF基因以及几个常见的O血清群密切相关。在分子流行病学研究中，PAI-USP亚型将提供属于系统发育组B2的大肠杆菌菌株的额外信息发现(Kanamaru S.等，Int.抗微生物药。对来自单纯性膀胱炎(UC)、复杂性膀胱炎(CC)和复杂性无症状性菌尿(CASB)患者的大肠埃希菌分离株的基因和血清学特性进行了检测。在所有类群中，系统发育类群B2占优势。18个毒力因子基因中的14个在三个类别中的流行情况相似，而PAP、IHA、ompT和PAI在UC相关分离株中比CC或CASB更常见(Takahashi A等，J.Clin。微生物。2006，44：4589-4592)。较少

Using crystal violet binding assay, we examined the ability of biofilm formation in 194, 76, 107 isolates from uncomplicated acute cystitis, pyelonephritis and prostatitis, respectively. The prostatitis isolates showed significantly higher OD values than cyctitis and pyelonephritis isolates. Similarly, strains of serotypes O4 and O22, which were frequently isolated from prostatitis, exhibited significantly higher OD values than other strains. Further, when the 21 prostatitis solates were examined for expression of curli fimbriae, 8 of 12 strains showing high OD value but only 3 of 9 showing low OD value expressed curli fimbriae. These results suggest an association between acute bacterial prostatitis and biofilm formation (kanamaru S. et al, Int. J. Urol. 2006, 28 (S1): S21-S25).A total of 427 Escherichia coli isolates from uncomplicated UTI (194 cystitis, 76 pyelonephritis, and 107 prostatitis) and 50 fecal isolates were examined for the phylogenetic grouping and PAI-usp subtyping as … More well as the prevalence of virulence factors (VFs) and O serogroups. Both phylogenetic group B2 and usp-positive strains were equally predominant in cystitis, pyelonephritis and prostatitis. Furthermore, each PAI-usp subtype was shown to be closely associated with several VF genes as well as several common O serogroups of UPEC. In molecular epidemiological studies, PAI-usp subtyping will provide additional informative findings of E. coli strains belonging to phylogenetic group B2 (Kanamaru S. et al, Int. Antimicrob. Agent 2006, 13: 754-760).The genetic and serological characteristics of Escherichia coli isolates from patients with uncomplicated cystitis (UC), complicated cystitis (CC), and complicated asymptomatic bacteriuria (CASB) were determined. Phylogenetic group B2 was predominant in all categories. The prevalences of 14 out of 18 virulence factors genes were similar among the three categories, while pap, iha, ompT, and PAI were more frequently seen in isolates associated with UC than CC or CASB (Takahashi A et al, J. Clin. Microb. 2006, 44: 4589-4592). Less