Toll-like receptor expression in SLE -new therapeutic approach for lupus-

目的探讨toll样受体（TLR）家族成员在系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中的表达。方法：我们分析了14例SLE患者和15例健康受试者的PBMC。流式细胞术检测PBMC细胞表面TLR2、TLR4的表达及细胞内TLR9的表达。结果：尽管健康受试者与SLE患者CD14+单核细胞上TLR4的表达无显著差异，但SLE患者单核细胞上TLR4的表达较健康受试者降低。SLE患者细胞内CD19+ B淋巴系统TLR9表达水平显著升高。然而，浆细胞样树突状细胞TLR9的表达水平在这些患者和健康人之间没有显著差异。结论：我们的研究结果表明，人外周血B细胞表达TLR9，且其在SLE患者中的表达增加。B细胞中TLR9的上调可能与SLE患者B细胞异常亢进有关。

To investigate expression of members of the Toll-like receptor(TLR) family in peripheral blood mononuclear cells(PBMC) in patients with systemic lupus erythematosus(SLE) . METHODS : We analyzed PBMC from 14 patients with SLE and 15 healthy subjects. The surface expressions of TLR2 and TLR4 and intracellurar expression of TLR9 on PBMC were analyzed by flow cytometry. RESULTS : Although TLR4 expressions on CD14+ monocytes were not significantly different between healthy subjects and patients with SLE, TLR2 expressions on monocytes were reduced in patients with SLE compared to healthy subjects. Intracellular TLR9 expression levels of CD19+ B lymphosytes were significantrly elevated in patients with SLE. However, the TLR9 expression levels of plasmacytoid dendritic cells were not significantly different between thrse patients and healthy subjects. CONCLUSION : Our results show that human peripheral blood B cells express TLR9 and that its expression is incressed in patients with SLE. This upregulated of TLR9 in B cells may be related to the abnormal B cell hyperactivity in patients with SLE.