New experimental pancreatic cancer model using real-time in vivo imaging system

1. PGHAM-1-Luc细胞的体外特性我们建立了一种新的细胞PGHAM-1-Luc，它是由AAV载体转导的具有荧光素酶活性的PGHAM-1细胞。PGHAM-1-Luc-1是PGHAM-1-Luc细胞中荧光素酶活性最高的5个亚克隆。荧光素酶活性通过IVIS成像系统（IVIS 100，Xenogen）用荧光素测定。PGHAM-1-Luc-1的倍增时间为18.2小时，比原始PGHAM-1.2的倍增时间长。使用PGHAM-1-Luc-1细胞的体内移植实验PGHAM-1的三种模型; 1）通过胰腺内移植的原发性胰腺癌和同时的肝转移，2）通过脾内移植的单独肝转移，3）通过腹膜内移植的腹膜播散，在移植后21天内。在14-21天，在原发性和转移性肿瘤中显示生物发光图像。脾内移植模型：脾内可见生物发光，未诱导肝转移;腹腔内移植模型：腹膜内可见极少量散在结节，生物发光微弱。从以上数据可以看出，PGHAM-1-Luc-1的转移活性低于PGHAM-1。肿瘤病理PGHAM-1-Luc-1移植的胰腺肿瘤组织学为中、低分化腺癌。PGHAM-1是分化良好的一种。任何变化都是通过基因诱导来实现的。

1. Characterization of PGHAM-1-Luc cells in vitroWe developed a new cells named PGHAM-1-Luc which is special PGHAM-1 cells with luciferase activity transduced by AAV vector. PGHAM-1-Luc-1 has most activity of luciferase among five subclones from PGHAM-1-Luc cells. Luciferase activity is measured by IVIS Imaging System (IVIS 100, Xenogen) with luciferin. Doubling time of PGHAM-1-Luc-1 was 18.2 hours that is longer than that of original PGHAM-1.2. In vivo transplantation experiment using PGHAM-1-Luc-1 cellsThree models for PGHAM-1 ; 1)primary pancreatic cancer and simultaneous liver metastasis by intrapancreatic transplantation, 2)liver metastasis alone by intrasplenic transplantation, 3)peritoneal dissemination by intraperitoneal transplantation, within twenty-one days after transplantation.Results of transplantation of PGHAM-1-Luc in hamsters.Intrapancreatic transplantation model : Bioluminescence image was shown in primary and metastatic tumors at 14-21 days. The sin of bioluminescence was almost same as actual size at autopsy.Intrasplenic transplantation model : Bioluminescence was found in spleen without induction of liver metastasis.Intraperitoneal transplantation model : Very low number of disseminated nodules on peritoneum with faint bioluminescence.From above data, the metastatic activity of PGHAM-1-Luc-1 was less than that of PGHAM-1.3. Pathology of tumorsHistopathology of pancreatic tumor by PGHAM-1-Luc-1 transplantation is moderately of poorly differentiated adenocarcinoma. PGHAM-1 is well-differentiated one. Any changes would be induced through gene induction.