Development of Thin Resin Film-BMP Composite

本项目的目的是寻找一种方法，利用骨形成蛋白和/或宿主来源细胞形成与上述形状相同的支架，在体内诱导足够适应骨缺损区形状的新骨，并将其植入活体组织中。本项目已成功研制出具有诱导新骨作用的EMA树脂-BMP复合材料、树脂材料形成的三维形态检测以及利用GST标记基因进行材料细胞毒性的快速筛选系统。在含有PLGA的新型树脂表面结合了骨形态发生蛋白。该系统显示了稳定的新骨形成，PLGA等载体作为骨形态发生蛋白的载体表现出良好的效果，并且可以替代糊精作为聚乳酸的载体。此外，通过高速X-…首次在世界范围内阐明了骨诱导的三维和随时间的变化更多的X线CT扫描，还显示了以骨形态发生蛋白为主要对象的薄层树脂膜新骨形成的三维图像。将荧光素酶基因与GST共转染到培养细胞中检测细胞毒作用，建立了荧光素酶基因自发光生物检测体系，取得了令人瞩目的成果。然而，在EMA树脂上培养细胞效果不佳。采用本组常用的未分化骨骼肌器官培养系统进行同种培养。最终，该系统也显示出组织的聚集能力较差。尽管如此，这些实验的信息对今后的工作非常重要，EMA树脂体系中可能包含的潜在分子可能会导致抑制间充质组织的增殖。通过该项目获得的系统，如含有骨形态发生蛋白的三维支架，将有助于开发新的组织工程方法，该项目将从现在起继续进行实验。较少

The objective of this project is to seek for the method to induce new bone in vivo which is adequately adapted to the shape of bone defect area by using scaffolds which has capability to form the same shape as mentioned as above with BMP and/or host origin cells, then implanted into the living tissue. The project has succeeded to make the EMA resin-BMP composites which induce new bone, three dimensional detection of the shape which was formed by resin material and rapid screening system for the cytotoxicity of materials by using the marker gene of GST. On the surface of the newly innovated resin that is containing PLGA, BMP was combined. This system revealed the stable new bone formation, and carrier such as PLGA showed excellent result as carrier of BMP, and it was also proven to be able to substitute the dextrin for the polylactic acid. Moreover, three dimensions of the bone induction and changes the passing time were clarified for the first time in the world wide by a high-speed, X- … More ray CT scan and also showed the 3-D image of new bone formation by thin resin film with BMP which is the main object of this experiment. Establishment of the bioassay system of self-luminescence using luciferase gene co-transfected with GST into the cultured cells to detect the cytotoxyty is remarkable achievement. However, to cultivate cells on the EMA resin could not have good result. Same kind of cultivation was performed by the organ culture system of undifferentiated skeletal muscle, which has been commonly experimented in this group. Eventually, this system also showed poor aggregation of tissues. Nonetheless, the information of these experiments showing was extremely important for the future work and the possible potential molecules containing in the EMA resin system may cause the inhibition of the mesenchymal tissue proliferation. Systems obtained through this project, such as 3 dimensional scaffolds with BMP, will attribute to developing new tissue engineering methods, and this project will be continuously experimented from now on. Less