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Development of Biomaterials which Induce Tissue and Organ

组织器官诱导生物材料的开发

基本信息

批准号：
12307047
负责人：
KAWAI Tatsushi
金额：
$ 7.4万
依托单位：
School of Dentistry Aichi-gakuin university
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12307047/
关键词：
BMP Bone morphogenetic protein scaffold biomaterial functional material implant osteoinduction インプラント代替骨

项目摘要

It is elucidated that both HAP-BMP composite material, which was used as a frame works, and artificial membrane - BMP composite material induced new bone and cartilage , those are useful as tissue organ inducible biomaterials. However, outer shape of implant, which was constructed with BMP as a framework, should have smooth surface and no sharp edge. Therefore, lubricating the surface of HAP and delete of the sharp edge is remarkably important. It is very hard to induce new bone inside of the inner vacant space of tubules shape of HAP. It demonstrates that not only the BMP is a factor of bone induction but also another unknown factor influence the osteogenesity. VEGF(vascular endothelial growth factor) or some other factors could be a cofactor for the new bone formation.It is considered that this information contributes largely to the next project. All artificial membrane -BMP composite showed new bone formation, and those are applicable for the clinical usage, such as GTR method. When biodegradable membrane is used, induction of new cartilage in vitro was very hard. More basic research work is necessary on this material for the application to clinical usage. There was only one research report for the cartilage induction on the artificial membrane previously. New cartilage formation on acetate membrane was successfully performed in this project. This induction remained only cartilage stage and not progress to osteoinduction but new bone formation could be possible by transplanting this system in vivo. Consequently, two kinds of tissue organ biomaterials, which are HAP-BMP composite and artificial membrane - BMP composite, are developed through these experiments, and important information for the application of this material for the clinical usage was accumulated.

结果表明，以HAP-BMP复合材料为骨架材料和人工膜-BMP复合材料均可诱导新生骨和软骨，可作为组织器官诱导材料。然而，以BMP为骨架构建的种植体外外形应表面光滑，无尖锐边缘。因此，对HAP表面进行润滑和去除锋利的边缘是非常重要的。在HAP小管状的内部空腔内很难诱导新生骨。这表明BMP不仅是骨诱导因子，而且是影响成骨的另一个未知因子。VEGF（血管内皮生长因子）或其他一些因子可能是新骨形成的辅助因子。人们认为这些信息对下一个项目有很大的帮助。所有人工膜-BMP复合材料均能形成新骨，适用于临床应用，如GTR法。当使用生物可降解膜时，体外诱导新软骨非常困难。该材料的临床应用还需要更多的基础研究工作。在人工膜上诱导软骨的研究，以往仅有一篇报道。本项目成功地在醋酸膜上形成了新的软骨。这种诱导仅停留在软骨阶段，而没有进展到骨诱导，但通过在体内移植该系统可以形成新的骨。因此，通过这些实验，开发了两种组织器官生物材料，即HAP-BMP复合材料和人工膜-BMP复合材料，为该材料的临床应用积累了重要的信息。