Development of BMP-Metal composite

为了研制金属-骨形态发生蛋白复合材料，研究了骨形态发生蛋白在金属表面的吸附情况，并将一些金属与骨形态发生蛋白结合起来评价其诱导成骨活性。用杆状病毒感染的SF-9细胞合成重组BMP，并插入BMP基因。然而，重组BMP的诱导成骨活性仅为天然来源BMP的1/10，其他系统也进行了研究，但所有重组BMP都具有微弱的诱导成骨活性。相反，从牛骨中提取水溶骨形成蛋白的方法稳定建立，提取出的骨形态发生蛋白含量稳定。在骨形态发生蛋白的纯化过程中提取了一种未知蛋白，经氨基酸分析确定为骨连接蛋白。由于骨骼中包含的其他蛋白质可能是BMP的天然载体，因此该蛋白质的纯化正在进行中。初步实验表明，纯钛与BMP具有良好的亲和力。对纯钛表面进行了阳极氧化处理，明胶载体的试件具有明显的骨诱导活性。喷砂实验组也表现出明显的高成骨活性，高纯度BMP或重组BMP的产生以及金属的表面处理将成为BMP临床研究的主要对象。

In order to develop the metal-BMP composites, absorb of the BMP to the surface of metals were investigated, then some metals and BMP were combined together to estimate its osteoinductive activity. Recombinant BMP was synthesized by sf-9cells infected by Baculo virus, which was, inserted the BMP gene. However, the osteoinductive activity of the recombinant BMP was 1/10 of BMP which was extracted from natural resource, Other system was also investigated, but all recombinant BMP showed faint osteoinductive activity. On the contrary, the method of extraction of water soluble BMP derived from bovine bone was established steadily and stable amount of BMP was being extracted. An unknown protein was extracted during the purification of BMP and it was determined as osteonectin by the amino acid analyses. As other proteins contained in bone may act natural carrier of BMP, the purification of that protein is on the experiment. Pure titanium showed excellent affinity to BMP as preliminary experiment. Anodic oxdized treatment was performed on the surface of pure titanium and the specimen with gelatin carrier was estimated remarkably high osteoinductive activity. Sand blasted experimental group showed also significantly high osteogenesity, Generation of highly purified BMP or recombinant BMP and the surface treatment of metals will be main subject of BMP for the clinical investigation.