Development of the noble biomaterial for re-implantation to host consisiting of chondrocyte culture system and titanium system

将宿主来源的软骨细胞培养体系与BMP-生物材料复合体系相结合，以促进软骨细胞的生物学活性。实验主要是在多孔钛支架上进行的。稳定生产了多孔钛和骨形态发生蛋白，成功完成了多孔钛的培养工作。骨形态发生蛋白-多孔钛复合体系有新骨形成，软骨细胞-多孔钛体系在体内诱导软骨形成，但没有新骨形成。新建立的扩散室系统不是在体外系统而是体内培养系统中培养细胞。该系统显示房间内有大量钙化。如上所述，本项目的主要目标得以顺利实现，相关研究工作提出了新的发现，涉及钛的薄表面层氧化钛的生物效应，以及DNA微阵列系统对分子生物学理解的有效性。基于这些有价值的信息的未来工作将在下一个项目中进行。

Host derived chondrocyte culture system and BMP-biomaterial composite system were integrated to promote the biological activity of chondrocyte. Experiment was performed mainly on the basis of porous titanium frame work. Porous titanium and bone morphogenetic protein was stably produced, and the culture work on porous titanium was successfully completed. New bone formation was observed by BMP-porous composite system, and chondrocyte-porous titanium system induced cartilage in vivo but no new bone formation. Diffusion chamber system was newly established to culture cells not in the in vitro system but inner body culture system. This system showed large amount of calcification in the chamber. As mentioned as above, primarily objectives of this project was successfully performed, and related research works raised new findings which were concerned with biological effect of titanium oxide, which is the thin surface layer of titanium, and with effectiveness of DNA micro array system for the molecular biological understanding. The future work based on these valuable information will be carried out in the next project.