Osteoinduction derived from cartilage, which induced on a composite polymer membrane with BMP

本实验进行了骨形态发生蛋白的大规模提取、支架材料的选择、膜材料上的软骨诱导以及诱导后的软骨在宿主体内的再植入。骨形态发生蛋白的提取已稳定上线，每月至少可提取骨形态发生蛋白1000 mg。考虑到BSE问题，将BMP的起始原料从牛骨改为猪骨。猪骨形成蛋白和牛骨形成蛋白的成骨能力无明显差异。对具有体内和体外生物学功能的聚合物进行了筛选。候选聚合物有胶原蛋白、明胶、琼脂糖凝胶、多糖、聚乳酸-PLGA共聚物、聚甲基丙烯酸乙酯和聚四氟乙烯，几乎所有聚合物都表现出优异的骨形态发生蛋白载体性能，最终选择聚四氟乙烯作为成膜材料。聚乳酸-聚丙交酯共聚物、聚甲基丙烯酸乙酯和甲基纤维素将是今后这一研究项目的重要材料。对多孔钛也进行了研究，结果显示钛孔内有良好的成骨细胞生长。骨形态发生蛋白诱导因子在聚四氟乙烯膜上形成软骨。将该软骨系统植入大鼠皮下组织内，3周后诱导成骨。通过这一结果，实现了本项目的目标。

Extraction of BMP in large scale, selection of scaffold, cartilage induction on a membrane material and osteoinduction in the host body by re-implant of induced cartilage were experimented. Extraction of BMP is on the line in stable, and at least 1000mg of BMP is obtained per month. Considering BSE problem, starting material of BMP was changed to pig bone from bovine bone. There was no difference between pig and bovine BMP in the ability of bone induction. Selection of polymers showing biological function both in vivo and in vitro was performed. Candidates of polymers were attelo collagen, gelatin, agarose, poly-saccharide, PLA-PLGA co-polymer, polyehylmethacrylate and polytetrafluoroethylene.Almost all polymers showed excellent property as BMP carriers, eventually, polytetrafluoroethylene was selected as a membrane forming material. PLA-PLGA co-polymer, polyehylmethacrylate and methylcellulose will be important material for this research project in future. Porous titanium was also investigated, and showed that osteoprogenitor cells were well harvested in the titanium pore. Cartilage formation was occurred on the polytetrafluoroethylene membrane by inducing factor of BMP. This Cartilage system was implanted into subcutaneous tissue of rat, which cause the osteoinduction at 3 weeks after implantation. The objective of this project was then achieved by this result.