EVALUATION OF GRANDE OF MALLIGNANCY ON THE BASIS OF CELL CYCLE ADHESION MOLECULESIN ORAL SQUAMOUS CELL CARCUNOMA

肿瘤抑制基因P53的异常调节在多种人类恶性肿瘤的发生发展中起着重要作用。一些研究人员认为，P53基因蛋白的累积表达可作为口腔鳞癌(OSCC)的预后指标，尽管另一些研究人员报道了相互矛盾的结果。本研究旨在探讨P53基因蛋白表达与140例口腔鳞癌临床病理特征的关系。对口腔鳞癌患者的肿瘤活检组织进行p53基因产物的免疫组织化学检测，特别是与临床病理结果和总生存期相关的表达模式。P53核聚集呈边缘型、弥散型或混合型。简单地评估P53的表达(阴性和阳性)，以10%的值定义，并不能提供关于口腔癌评估的有用信息。然而，Depe…Rsed-p5 3蛋白高表达与淋巴结转移、肿瘤细胞分化程度、总生存期和突变率显著相关。这些发现表明，P53的表达，而不是P53的阳性，可能是口腔鳞癌恶性程度的生物学标志之一。抑癌基因的遗传和表观遗传改变在人类肿瘤发生中发挥着重要作用。RAS信号在口腔鳞状细胞癌中经常被激活，尽管在口腔鳞癌患者中很少检测到RAS突变。在本研究中，我们检测了RAS相关家族(RASSF)基因在口腔鳞癌中的表达和甲基化状态。RASSF1(7/17：41.2%)、RASSF2(12/17：70.6%)、RASSF5(4/17：23.5%)在一组口腔鳞癌细胞系以及46例口腔鳞癌患者(RASSF1，6/46，13.0%；RASSF2，12/46，26.1%；RASSF5，5/46，10.9%)中检测到甲基化。我们的研究结果表明，RASSF的表观遗传失活在口腔鳞癌的发生发展中起着关键作用，RASSF可能是口腔鳞癌诊断和治疗的重要分子靶点。较少

Dysregulation of tumor suppressor gene p53 has been shown to play a significant role in development of a wide variety of human malignancies. Some investigators have suggested that accumulated expression of p53 gene protein is useful as a prognostic indicator in oral squamous cell carcinoma (OSCC), although others have reported conflicting result. The aim of this study is to determine the correlation between the expression of p53 gene protein and the clinicopathological features of 140 OSCC. Tumor biopsies obtained from patients with OSCC were examined for the immunohistochemical detection of p53 gene product with special reference to expression pattern correlated with clinicopathological findings and overall survival. Nuclear accumulation of p53 was visualized as marginal pattern, dispersed pattern, or mixed pattern. Simple evaluation of p53 expression (negative and positive) defined with value of 10% did not provide useful information as to the assessment of oral.cancer However, dispe … More rsed type p53 protein expression significantly correlated with nodal metastasis, differentiation of tumor cell, overall survival and mutation. These finding suggest that p53 expression may, but not p53 positivity, be one of biological makers for the degree of malignancy in OSCC.Genetic and epigenetic alterations in tumor-suppressor genes play important roles in human neoplasia. Ras signaling is often activated in OSCC, although RAS mutations are rarely detected in OSCC patients. In present study, we examined the expression and and methylation statuses of RAS association family (RASSF) genes in OSCC. We frequently detected methlaion of RASSF1 (7/17:41.2%) RASSF2 (12/17:70.6%), RASSF5 (4/17:23.5%) in a panel of OSCC cell lines, as well as in specimens collected from 46 OSCC patient (RASSF1, 6/46, 13.0%; RAsSF2,12/46, 26.1%; RASSF5,5/46,10.9%). Our findings indicate that epigenetic inactivation of RASSF plays a key role in OSCC tumorigenesis, and that RASSF may be an important molecular target for the diagnosis and treatment of OSCC. Less