Studies on the SUMO modification system : Towards understanding regulation of the nuclear function and structure

小的泛素相关修饰物2/3(SUMO2/3)可以在翻译后与构成染色质的多种蛋白质连接，染色质是遗传和表观遗传调节的平台。然而，目前尚不清楚SUMO2/3和SUMO2/3修饰蛋白是如何传递到染色质纤维上的。我们报道了染色质组装因子1(CAF-1)的最大亚基-人P150，它在DNA复制的背景下促进染色质组装，直接并优先与SUMO2/3相互作用。我们的发现表明P150作为SUMO2/3相互作用因子的作用扩大，并提出了P150在促进复制过程中染色质纤维上SUMO2/3或SUMO2/3修饰蛋白(或两者)递送的有趣可能性。

The small ubiquitin-related modifier 2/3 (SUMO2/3) can be post-translationally conjugated to a wide variety of proteins constituting chromatin, the platform for genetic and epigenetic regulation. Nevertheless, it is unclear how SUMO2/3 and SUMO2/3 modified proteins are delivered to the chromatin fibers. We report that the largest subunit of chromatin assembly factor 1 (CAF-1), human p150, which, in the context of DNA replication, promotes chromatin assembly, interacts directly and preferentially with SUMO2/3. Our findings suggest an expanded role of p150 as a SUMO2/3-interacting factor, and raise the intriguing possibility that p150 plays a role in promoting delivery of SUMO2/3 or SUMO2/3 modified proteins (or both) on chromatin fibers during replication.