Study of the mammalian G1 regulatory mechanism unifying Rb and p53 pathways.

我们研究了哺乳动物细胞周期G1期进展的调节机制，重点是COP 9信号体复合物如何调节Rb和p53通路。我们发现COP 9信号体的第五亚基（CSN 5）通过与组蛋白甲基转移酶SMYD 3相互作用，与p16基因的启动子区结合并调控其转录，表明CSN 5、SMYD 3、p16、cyclin D-Cdk 4/6和Rb组成的信号通路在造血干细胞的调控中起重要作用。我们还发现，第三个组件，CSN 3，通过MLF 1参与COP 1-p53通路的调节。

We studied the regulatory mechanism of G1 progression of the mammalian cell cycle by focusing on how the COP9 signalosome complex regulates Rb and p53 pathways. We found that the fifth subunit of the COP9 signalosome(CSN5), through interaction with a histone methyltransferase, SMYD3, bound to the promoter region of the p16 gene and regulated its transcription, indicating that the signaling pathway consisting of CSN5, SMYD3, p16, cyclin D-Cdk4/6, and Rb plays an important role in regulation of the hematopoietic somatic stem cells. We also found that the third component, CSN3, participated in the regulation of the COP1-p53 pathway through MLF1.