Establishment of novel preventive/therapeutic measures and demonstration of a mechanism of neural spread of pathogens in neurotropic zoonotic diseases

通过鞘内接种(将接种抗原接种到蛛网膜下腔)在脑脊液中检测到的特异性抗体在大脑中从头产生。鞘内接种完全阻止了接种狂犬病或伪狂犬病病毒的小鼠的临床症状和死亡。鞘内接种和脑内直接接种狂犬病病毒的小鼠存活率达80%，表明鞘内接种适用于狂犬病动物和人的治疗。我们新的儿童流感脑病小鼠模型提示，流感病毒感染和/或流感病毒感染和内毒素血症的重复引起的高细胞分裂素血症可能是该病的原因(S)。

Specific antibodies detected in cerebrospinal fluid by intrathecal vaccination (vaccination by an inoculating antigen into subarachnoidal space) were produced de novo in the brain. Intrathecal vaccination completely prevented clinical signs and death of mice inoculated with rabies or pseudorabies viruses. Eighty percent of the mice intrathecally vaccinated and inoculated rabies virus directly into the brain survived, and this result suggested that the intrathecal vaccination was applicable for the treatment of rabid animals and humans. Our novel murine model of influenzal encephalopathy of childhood suggested hypercytokinemia triggered by influenza virus infection and/or a duplication of influenza virus infection and endotoxemia might be the cause(s) of the disease.