Basic research objected to establish the highly qualified assessment of hepatic reserve function by utilizing new molecular marker localized in liver

这些研究主要包括以下三个方面的内容：（1）用于肝功能评价的低定位分子的研究;（2）被认为具有关键作用的肝干细胞的分离、鉴定和增殖的调控;（3）基因转染肝细胞或肝素干细胞治疗肝功能障碍的可能性。结果表明：（1）去唾液酸糖蛋白受体计数方法和粘附分子（尤其是claudins）的分布对肝细胞功能的逻辑意义;（2）肝干细胞的体外修饰成功;（3）利用基因转染的肝细胞或新鲜肝干细胞进行细胞移植的临床能力。

The researches were composed of three kinds of contents as follows : (1) the studies about low localized molucules utilized for the assessments of hepatic function, (2) the regulation on isolation, identification and proliferation of hepatic stem cells recognized to have key roles and (3) the possibility of gene-transfected hepatocyte or heparic stem cell on the treatment for hepatic dysfunction. Our results showed (1) the logical significance of asialoglycoprotein receptor count method and the distribution of adhesion molecules (especially claudins) on hepatic cell function, (2) successful modification of hepatic stem cells in vitro, and (3) the clinical capability by the cell transplantation utilized gene-transfected hepatocytes or fresh hepatic stem cells.