The interaction between adipose tissue and tubular epithelium on their proliferation, polarization and differentiation

产生脂肪因子的脂肪组织，由前脂肪细胞、脂肪细胞和间充质干细胞组成，环绕在肾脏周围。为了研究肾小管细胞与脂肪组织的相互作用，我们将脂肪组织片段与MDCK细胞共培养。共培养的MDCK细胞比单培养的MDCK细胞分化较好。当我们在单独培养的MDCK细胞中加入瘦素时，脂肪组织诱导的形态学变化被复制。我们的研究表明，脂肪组织碎片通过对抗瘦素和脂联素的内分泌或旁分泌作用，通过抑制MDCK细胞的生长和凋亡，从而促进MDCK细胞的肥大、极化和分化。此外，MDCK细胞抑制脂肪组织中前脂肪细胞和间充质干细胞的再生。

Adipokine-producing fatty tissues, composed of preadipocytes, adipocytes, and mesenchymal stem cells, surround the kidney. To study the interaction between renal tubular cells and adipose tissue, we cocultured adipose tissue fragments and MDCK cells. MDCK cells in the coculture showed fine differentiation than that seen in MDCK cell monoculture. The adipose tissue-induced change in morphology was replicated when we added leptin to MDCK cells cultured alone. Our study shows that adipose tissue fragments promote the hypertrophy, polarization, and differentiation of MDCK cells by attenuating their growth and apoptosis through opposing endocrine or paracrine effects of leptin and adiponectin. Further, MDCK cells inhibit the regeneration of preadipocytes and mesenchymal stem cells in adipose tissue.