In-Situ Single-Molecule Fluorescence Imaging of Membrane Protein Interaction Using Nano-Structured Glass Plate

对于膜蛋白的原位单分子荧光成像，我们使用计算光学模拟研究了纳米波导的结构，然后在玻璃衬底上制作波导。我们研究了与波导结构有关的用于实时单分子成像的波导的可行性。我们已经开发出蚀刻波导和平面波导的制造工艺。我们发现，蛋白质-蛋白质相互作用可以用蚀刻波导荧光检测，并且蚀刻波导的信噪比是常规波导的六倍。虽然我们还没有成功地观察到人脑蛋白质相互作用使用palner波导，我们建立了基本的技术观察，并已成功地检测单分子荧光使用平面波导。

For in-situ single-molecule fluorescence imaging of membrane proteins, we investigated the structure of nano-waveguides using computational optics simulation, followed by fabrication of the waveguides on glass substrates. We studied the feasibility of the waveguides for real-time single-molecule imaging in relation to the waveguide structure. We have developped processes for the fabrication of both etched waveguides and planer waveguides. We showed that the protein-protein interaction can be fluorescently detected with the etched waveguide, and also that the signal-to-noise ratio of the etched waveguide is six times the conventional waveguide. Although we have not yet succeeded in observing menbrain protein interaction using the palner waveguide, we constructed the elemental techniques for the observation, and have succeeded in detecting the single-molecule fluorescence using the planer waveguide.