Functions of myeloid-lectin receptors

我们产生了SIGNR 3特异性mAb并研究了SIGNR 3在体内的表达。SIGNR 3在多种组织中的绝大多数CD 11b ^+ CD 11 c ^<low>髓样细胞上表达，如血液、真皮、肺、脾和淋巴结。通过使用荧光化的Ly 6C-<high>SIGNR 3-骨髓单核细胞的细胞转移实验，在循环期间，随着Ly 6C表达的降低，SIGNR 3表达沿着上调。在通过HEV到达外周淋巴结后，接种的细胞在几天内分化为CD 11b ^+ DC。这些结果表明，部分DC，尤其是皮下淋巴结中的DC，可能由Ly 6C ~+<high>单核细胞补充; DCIR 1主要存在于各种细胞类型的细胞内，但在LPS和TNF-α的激活下转移到细胞表面。

We generated SIGNR3-specific mAbs and investigated SIGNR3 expression in vivo. SIGNR3 was expressed on a vast majority of CD11b^+CD11c^<low> myeloid cells in various tissues, such as blood, dermis, lung, spleen and lymph nodes. By the cell transfer experiments using fluorescenated Ly6C^<high>SIGNR3^- bone marrow monocytes, SIGNR3 expression was upregulated along with the decrease in Ly6C expression during the circulation. Upon arrival at the peripheral LNs through HEV, the inoculated cells differentiated into CD11b^+ DCs in a couple of days. These results indicate that some DCs, especially in the subcutaneous lymph nodes, are possibly replenished by Ly6C^<high> monocytes.DCIR1 was detected mainly intracellularly in various cell types, but transferred onto the cell surface by the activation with LPS and TNF-α.