Estimation of animal experiment models to evaluate the early inflammation stage in adipocytes following obesity and investigation of anti-inflammatory factors in foods

研究了高脂或低脂饮食和喂养时间对瘦素受体缺陷异型C57BL/6J、KK/Ta、b6db/+小鼠脂肪细胞炎症参数基因表达的影响。结果表明，饲喂高脂饲料4周后，大鼠腹部脂肪量明显增加，炎症指标的mRNA表达明显增加。短期喂养时，可检测到的炎症指标为NADPH氧化酶亚基、MCP-1、IL-6、抵抗素。在喂养8周或12周时，观察到糖耐量受损和脂肪细胞增大。利用这些参数，观察大麦和乳钙的抗炎作用。

We investigated the influences of high fat or low fat diet and feeding periods on the mRNA expressions of adipocyte inflammatory parameters in C57BL/6J, KK/Ta, B6db/+(leptin receptor deficient hetero type) mice. Our results indicated that significant increase in abdominal fat mass and mRNA expressions of inflammatory parameters were observed after 4 weeks feeding of the high fat diet. On the short term feeding, detectable inflammatory parameters were NADPH oxidase subunits, MCP-1, IL-6, resistin. Impaired glucose tolerance and adipocyte enlargement were observed on the 8 or 12 weeks feeding. Using these parameters, anti-inflammatory effects of barley and dairy calcium were observed.