Studies on the biological effects and mode of action of pollutants in marine animals

我们获得了暴露于有机锡化合物、金属离子或内分泌干扰物72小时后海鞘的基因表达谱数据。然后，我们使用我们的分类方法对每种污染物的上调或下调基因进行分类，并估计每种污染物的生物效应。我们建立了高通量筛选海鞘卵和幼虫胚胎发生和变态的方法，并分析了每种污染物的抑制作用。此外，我们还发现了9个用于监测有机污染物的标记基因。

We obtained gene expression profile data from ascidians after exposure to organotin compounds, metal ions, or endocrine disruptors for 72h. We then categorized the up- or down-regulated genes for each pollutant using our classification method, and estimated the biological effects of each pollutant. We developed high-throughput methods for screening embryogenesis and metamorphosis using ascidian eggs and larva, and analyzed the inhibitory effects of each pollutant. Furthermore, we found nine marker genes for monitoring environmental contamination due to organotins.