Development of enrichment methods of sialoglycopeptides towards glycobiomarker discovery

糖蛋白作为一种新型的临床生物标志物具有很大的潜力。我们研究了一种新的唾液酸糖蛋白的富集法，作为发现唾液酸糖蛋白生物标记物的途径。经过大量的实验，我们发现生成肟基是唾液酸糖蛋白与树脂珠偶联的最实用的方法之一。然后，我们设计并合成了一种能够在唾液酸侧引入羟胺基团的连接体。

Glycoproteins have great potential as novel clinical biomarkers. We studied on the development of a novel enrichment method of sialoglycoproteins which contain sialic acids on their glycan moieties, as an approach to biomarker discovery focused on sialoglycoproteins. After intensive trials, we found that oxime formation is one of the most practical methods for coupling of sialoglycoproteins and resin beads. Then, we designed and synthesis of a linker which can introduce oxyamine group at sialic acid moiety.