Preparation of the glycol-arrays for aiming at the ordered and well-defined accumulation and the analyses of the interaction to proteins

为了阐明碳水化合物与蛋白质之间的多价相互作用，制备固定在固体表面的乙二醇阵列，我们利用生物素-亲和素相互作用成功地在表面上分散排列了碳水化合物配体，并利用QCM系统进行了蛋白质结合的动力学分析。此外，我们还分析了verotoxin（一种负责细胞表面o-157和Gb3糖分子的物质）之间的相互作用力。通过改变加载速率得到断裂力，得到有效键长，这是分析相互作用机理的重要参数。我们可以检测到单分子相互作用的力，我们将通过对力的分析更详细地讨论相互作用的机理。

In order to elucidate the multivalent interactions between carbohydrates and proteins, and to prepare the glycol arrays which immobilized onto the solid surfaceWe could succeed in arraying carbohydrate ligands on the surface with dispersion by utilizing the biotin-avidin interaction, and the kinetic analyses for protein binding could be performed with the QCM system.Moreover, we have analyzed interaction forces between a verotoxin that is a substance responsible for o-157 and Gb3 sugar molecules on a cell surface. The rupture forces were obtained by changing loading rates and we can get an effective bond length which is an important parameter for an analysis of interaction mechanisms. We can have detected single-molecular interaction forces, and we will discuss about the interaction mechanism in more detail by an analysis of the forces.