Development of Nano Device to Detect Cellular Stress by Using Supercritical Coating method.

在本工作中，我们合成了含有氨基苯甲酸的多肽作为超临界二氧化碳中的荧光探针，以检测具有降压作用的血管紧张素转换酶抑制剂。用合成的荧光肽进行了细胞应激检测。通过心肌细胞实验观察血管紧张素转换酶抑制剂对细胞应力和血管扩张的影响。此外，为了寻找控制高血压的物质，利用合成的荧光肽对豆浆和豆腐(与大豆有关的食品)中的目标物质进行了分离和纯化。

In this work, we synthesized the peptides including an amino benzoic acid group as fluorescent probe in supercritical carbon dioxide in order to detect ACE inhibitor which reduces hypertension. The cellular stress detection was tried by using this synthesized fluorescent peptide. The effect of ACE inhibitor on the reduction of the cellular stress and blood vessel dilation was experimentally examined by means of cardiomyocyte. Furthermore, in order to search the hypertension control substances, separation and purification of target substances included in the soybean milk and the tofu (food related to the soybean) were carried out by using the synthesized fluorescent peptide.