Structural change of telomere DNA induced by radical reaction

端粒存在于真核生物的染色体末端，由端粒DNA和端粒结合蛋白组成，端粒DNA含有丰富的鸟嘌呤碱基的重复序列。通过停流-ESR和核磁共振研究端粒序列，并产生超氧阴离子自由基和单线态氧，验证了端粒序列的损伤。此外，还研究了亚硝酸对端粒DNA的损伤作用，并考察了端粒DNA与蛋白质的交联反应。

Telomere that exists in eukaryote's chromosome terminus is composed of telomeric DNA with the repetitive sequence to which the guanine base is abundant and of telomere binding proteins. The damage of the telomere sequence was verified by researching this telomeric site by using stopped-flow-ESR and NMR and generating superoxide anion radical and singlet oxygen. In addition, telomere DNA damage with the nitrous acid was concurrently studied, and the cross-linking reaction with the protein was also examined.