Molecular basis for the formation of translational repression complex that regulates stem cell differentiation

Musashi1（Msi1）是一种神经蛋白，抑制靶mRNA的翻译，并有助于维持细胞干细胞和肿瘤发生。我们已经确定了Msi1的RNA识别机制。Msi1的最小RNA序列以及我们所阐明的Msi1的结合机制将为发现Msi1的新靶基因提供重要线索。同时，我们确定了PABP的结构，并确定了其Msi1结合位点。最后，我们准备了一个建立蛋白质和核酸复合物模型的协议，这将支持翻译抑制复合物的结构分析。

Musashi1 (Msi1) is a neural protein that suppresses translation of target mRNAs and contributes in maintenance of cell stemness and tumorigenesis. We have determined the RNA recognition mechanism by Msi1. Minimal RNA sequence for Msi1, and the binding mechanism of Msi1 that we have elucidated would provide important clues to discover new target genes for Msi1. Meanwhile, we have determined the structure of PABP and identified its Msi1 binding sites. Finally, we have prepared a protocol for building protein and nucleic acid complex models that would support the structural analysis of translational repression complex.