Development of a culture system to selectively induceγ δT-lymphocytes from embryonic and hematopoietic stem cells

为了建立一种选择性诱导小鼠gd T淋巴细胞分化的培养体系，首先对几种基质细胞系中gd T淋巴细胞潜在配体MHC Ib类分子及其相关分子的基因表达进行了定量研究。根据实验结果，建立了高表达T10和T22抗原的基质细胞株作为首选。在单层基质细胞上，T淋巴细胞由胚胎干细胞诱导而来，但迄今尚未观察到T10和T22能诱导gdT淋巴细胞选择性和优势分化并影响其谱系。文化体系仍在进一步分析中。

In order to develop a culture system that can selectively induce the murine gd T lymphocyte differentiation, first, the gene expression of MHC class Ib and its related molecules as potential ligands of gd T lymphocytes in several stromal cell strains were quantitatively analyzed. From the results, stromal cell strains overexpressing T10 and T22 antigens as a first choice were established. On a single layer of the stromal cells, T lymphocytes were induced from embryonic stem cells but it has not observed the fact that T10 and T22 can induce the selective and predominant differentiation of gd T lymphocytes and influence their repertoire so far. The culture system is still being further analyzed.