Conversion of catalytic mechanism of glycoside hydrolases

我发现了一个独特的糖苷水解酶家族，它含有转化和保留催化酶。这种差异是由于催化残基位置的简单差异造成的。我们试图将这些酶的催化机理相互转换。然而，这一尝试失败了。这些结果表明，在进化的早期阶段，两个相似的酶从一个共同的祖先分化而来，每个酶以不同的方式进化。

I discovered an unique glycoside hydrolases family which contains inverting- and retaining-catalytic enzymes. This divergence was due to the simple difference of the position of catalytic residues. We attempted to convert the catalytic mechanism of these enzyme each other. However, the attempt was failed. These results indicated that two similar enzymes diverged from a common ancestor in early stage of evolution and each enzyme evolved in different ways.