Noninvasive assessment of the brain redox status in chronic kidney disease mouse using overhauser-enhanced magnetic resonance imaging
Noninvasive assessment of the brain redox status in chronic kidney disease mouse using overhauser-enhanced magnetic resonance imaging
批准号:
20790039
负责人:
YAMATO Mayumi
金额:
$2.75万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2009
中文摘要
尿素毒素可通过N-甲基-D-天冬氨酸(NMDA)受体的活性诱导谷氨酸能神经元损伤。在这项研究中,我们旨在利用Overhauser增强磁共振成像(OMRI)无创性地显示慢性肾脏疾病小鼠脑内氧化还原的变化。以氧化还原敏感的造影剂3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-l-oxyl的减少率作为体内氧化还原状态的指标。CKD发病后2周和4周,8-羟基鸟苷(8-OHdG)和记忆障碍无明显变化,但CKD发病4周后,由连续图像计算的甲氧羰基-丙氧基还原速率显著增加。在CKD发病8周后,我们观察到8-OHdG免疫阳性细胞数量增加和记忆障碍。因此,脑氧化还原改变的非侵入性成像与CKD引起脑损伤的早期阶段有关。
英文摘要
Urea toxin is suggested to be induced the glutamatergic neuronal damage mediated by the activity of N-methyl-D-aspartate (NMDA) receptor. In this study, we aimed to demonstrate brain redox alterations in chronic kidney disease mouse noninvasively, using overhauser-enhanced magnetic resonance imaging (OMRI).The reduction rate of 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-l-oxyl (methoxycarbonyl-PROXYL), a redox-sensitive contrast agent, was used as an index of the redox status in vivo. No changes were seen in the 8-OHdG (8-Hydroxydeoxyguanosine) or the memory disturbance at 2 or 4 weeks after CKD onset, but after 4 weeks of CKD, the methoxycarbonyl-PROXYL reduction rate, calculated from continuous images, had increased significantly. At 8 weeks after CKD onset, we observed an increase in the number of 8-OHdG-immunopositive cells and the disturbance in the memory. Thus, the noninvasive imaging of the brain redox alterations was associated with the initial stages of CKD cause brain injury.
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Analysis of brain dysfunction in HFDinduced obese mice
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批准号:22790039
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.5万
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财政年份:2010
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负责人:YAMATO Mayumi
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依托单位:
海外基金