Identification of molecular mechanism of excitement with inhalational anesthetics

本研究的目的是确定吸入麻醉药引起兴奋的分子机制。根据蓝斑神经元的膜电位记录，表明PKC参与了七氟醚引起的蓝斑兴奋。并制作了行为分析装置，对大鼠的行为进行数值表达。有趣的是，三种挥发性麻醉剂（七氟醚、异氟醚和氟烷）产生内向电流的顺序，特别是七氟醚的内向电流大于氟烷的事实，完全符合行为研究表明，七氟醚在这些麻醉剂中引起大鼠最强烈的躁动。七氟醚在LC神经元中激活的兴奋电流可能是七氟醚矛盾兴奋作用的潜在细胞机制之一。

The purpose of the present study is to identify the molecular mechanism of the excitement with the inhalational anesthetics. According to the membrane potential recordings of locus coeruleus neurons, it was shown that PKC took part in the excitement of locus coeruleus caused by sevoflurane. Moreover, the behavioral analysis device was made, and the behavior of the rat was expressed numerically. Interestingly, the order in generating inward current for three volatile anesthetics (sevoflurane, isoflurane and halothane), especially the fact that the inward current with sevoflurane was larger than that with halothane, is in complete accord with the behavioral studies showing that sevoflurane provokes the agitation at the strongest in the rat among these anesthetics. The excitatory current activated by sevoflurane in LC neurons might be one of the potential cellular mechanisms underlying paradoxical excitatory effect of sevoflurane.