Eotaxin Immunoreactivity in the Nasal Polyps of Eosinophilic Chronic Rhinosinusitis Patients by using Laser capture microscope

在鼻息肉中，eotaxin亚家族，eotaxin-1， -2和-3在大多数浸润性嗜酸性粒细胞以及其他炎症细胞，上皮细胞和内皮细胞中都有免疫反应。与非嗜酸性CRS组相比，嗜酸性CRS组的嗜酸性细胞中有显著的eotaxins表达。ELISA检测嗜酸性CRS组鼻息肉和鼻窦积液eotaxin浓度明显高于非嗜酸性CRS组。通过激光捕获显微解剖，从富含嗜酸性粒细胞的上皮下组织中扩增出eotaxin-3。

In the nasal polyps, immunoreactivities of the eotaxin subfamily, eotaxin-1, -2, and -3, were noted in most of the infiltrating eosinophils, as well as in other inflammatory cells, epithelial cells, and endothelial cells. Compared with non-eosinophilic CRS groups, eosinophilic CRS groups had a significant expression of eotaxins in their eosinophils. The eotaxin concentrations of nasal polyp and sinus effusion as measured by ELISA were significantly increased in the eosinophilic CRS group compared to the non-eosinophilic CRS group. By laser capture microdissection, eotaxin-3 alone was amplified from the eosinophil-rich subepithelial tissues.