The analysis of the interaction between folic acid and embryonic palatal fusion for the treatment of the patients with cleft lip with or without cleft palate

本研究旨在为今后在唇腭裂治疗中的应用奠定基础。利用小鼠模型，我检测了腭裂发生过程中基因表达的变化，细胞黏附分子Ceacam1被详尽地上调，所以我专注于这个基因。在没有这个基因的小鼠和通过功能阻断抗体抑制这一遗传功能的两种情况下，我发现腭裂发生延迟。此外，叶酸被认为对预防腭裂有效，发现当我让怀孕的小鼠从怀孕早期开始摄入叶酸时，胚胎腭部Ceacam1的表达增加。从这些结果来看，提示孕早期叶酸摄入增加了Ceacam1的表达，参与了早期腭裂的发生。

This study aims at the application to cleft lip/cleft palate treatment in the future. Using a mouse model, I examined a change of the gene expression in palatogenesis and cell adhesion molecules called Ceacam1 were up-regulated exhaustively, so I focused on this gene.In both cases of the mouse which did not have this gene and that inhibited this genetic function by function-blocking antibody, I found out that palatogenesis was delayed.In addition, folic acid was said to be effective to the prevention of the cleft palate, it was identified that expression of Ceacam1 of the embryonic palate increased when I let pregnant mice ingest folic acid from early gestation.From these results, it was suggested that intake of folic acid from early gestation was increase the expression of Ceacam1 and it was involved in early palatogenesis.