ROLE OF CALPAIN IN PATIENTS WITH RECURRENT MISCARRIAGE

探讨反复流产患者血清中抗钙蛋白酶抑制蛋白抗体的存在、作用及其与抗磷脂酰乙醇胺（PE）抗体的相关性。受试者组为有反复流产史的患者，对照组为知情同意的健康女性。我们使用中期子宫内膜进行免疫组织化学来研究钙蛋白酶和钙蛋白酶抑制蛋白的定位。采用ELISA法检测各组非妊娠期血清中抗钙蛋白酶抑制蛋白抗体和PE结合蛋白依赖性IgG抗体。中期子宫内膜间质细胞和上皮细胞胞浆中均可见μ-calpain和calpastatin染色，但染色方式无显著性差异。血清抗钙蛋白酶抑制蛋白抗体受试组为4.317±0.987（U/ml），对照组为3.373±0.653（U/ml）。实验组明显高于对照组。抗Calpastatin抗体与抗PE抗体之间无明显相关性。提示钙蛋白酶-钙蛋白酶抑制素系统可能独立于抗PE抗体-钙蛋白酶通路在反复流产子宫内膜中发挥重要作用。

We investigated the existence and contribution of anti-calpastatin antibody and correlation with antiphoshatidylethanolaine (anti-PE) antibody in serum of recurrent miscarriage. Subject group was patients with history of recurrent miscarriage and control group was healthy women with informed consent. We performed immunohistochemistry using mid-phase endometrium to investigate localization of calpain and calpastatin. ELISA was performed to measure anti-calpastatin antibody and PE-binding protein dependent IgG antibody in sera of non-pregnancy period in each group. Staining of μ-calpain and calpastatin were observed in the cytoplasm of stromal and epithelial cells in mid-phase endometrium but no significant differences were observed in staining patterns. Subject group of anti-calpastatin antibody in sera was 4.317±0.987 (U/ml) and control group was 3.373±0.653. Subject group was significantly higher than control group. There was no significant correlation between anti-calpastatin antibody and anti-PE antibody. The result provided that calpain-calpastatin system may plays important role in endometrium of recurrent miscarriage independently from anti-PE antibody-calpain pathway.