Anti-aging research on the oocyte reproductive function based on the gene expression profiling

线粒体作为细胞器存在于细胞中，参与氧化磷酸化作用，为细胞提供ATP。我们的研究表明，40岁以上女性的未受精卵和未卵裂胚胎mtDNA拷贝数明显偏低。线粒体DNA拷贝数与卵裂球细胞体积呈正相关。从胚胎形态学分类与胚胎发育的关系方面探讨了斑马鱼胚胎的质量。随着年龄的增长，形态差的胚胎发光时间延迟，胚胎发育效率有降低的趋势。从卵母细胞凋亡的角度出发，利用基因芯片技术研究了斑马鱼caspase基因的表达。通过caspase基因表达的增加揭示了胚胎的凋亡。

Mitochondria exists in cell as an organelle that takes part in the role of oxidative phosphorylation to supply ATP to the cell. Our studies demonstrated that lower mtDNA copy number existed in unfertilized oocytes and uncleavaged embryos originated from over 40 years of age women significantly. A positive correlation was demonstrated between mtDNA copy number and the cell volume of blastomere. We examined the quality of embryo in zebrafish, in terms of the implication between morphological classification of embryo and embryonic development. Glowing of poor form embryo was delayed and the efficiency of embryonic development tended to be reduced by aging. From the respect of apoptosis on the oocyte, microarray analysis was applied to caspase gene expression in zebrafish. Embryo was revealed in apoptosis through the increased expression of caspase genes.