The role of vascular endothelial HSP70 on acute lung injury

中性粒细胞的跨内皮迁移是急性炎症的关键步骤。在本研究中，估计热休克蛋白（HSP）的作用，transwell室迁移分析系统纳入内皮单层被用来检查迁移的中性粒细胞的数量。内皮血管舒张刺激磷蛋白（VASP），负责控制肌动蛋白丝的几何形状的蛋白质的表达，也估计在选定的时间在中性粒细胞迁移，在体外。结果表明，羟乙基淀粉（HES）预处理内皮细胞显著减少中性粒细胞的迁移，槲皮素（HSP 70抑制剂）抑制羟乙基淀粉对中性粒细胞跨内皮迁移的影响，VASP磷酸化通常与中性粒细胞跨内皮迁移的抑制有关。这些数据表明，内皮VASP通过调节与上游HSP 70信号转导机制相关的细胞骨架事件来调节中性粒细胞的跨内皮迁移。

The transendothelial migration of neutrophils is a critical step in acute inflammation. In the present study, to estimate the role of heat shock protein (HSP), a transwell chamber migration assay system incorporating endothelial monolayer was used to examine the numbers of migrating neutrophils. The expression of endothelial vasodilator-stimulated phosphoprotein (VASP), a protein responsible for controlling the geometry of actin-filament, was also estimated at selected times during the neutrophil migration, in vitro. The results showed that pretreating endothelial cells with hydroxyethyl starch (HES) significantly diminished neutrophil migration, quercetin (HSP70 inhibitor) inhibited the effect of HES on neutrophil transendothelial migration, and VASP phosphorylation normally associated with the inhibition of neutrophil transendothelial migration. These data suggest that endothelial VASP regulate transendothelial neutrophil migration by modulating the cytoskeletal events that associated with upstream HSP70 signal transduction mechanisms.