Development of sponge scaffold and osteogenic promoting factors in the scaffold for dentine-pulp complex regeneration

使用多孔陶瓷作为未分化间充质干细胞成骨的支架是可取的。在支架中，细胞可以向硬组织形成细胞活跃增殖和分化。因此，对影响细胞分化的因素进行了研究。结果表明，透明质酸、层粘连蛋白和亮氨酸能有效地促进细胞的增殖和分化。研究发现，葡聚糖对未分化间充质细胞的分化有一定的作用。陶瓷支架的毛孔中含有骨髓干细胞。然而，研究发现，由于纤维结构，骨髓干细胞不可能附着在具有纤维结构的海绵中。将葡聚糖包覆到海绵上可以增加纤维的宽度，这样就有可能附着骨髓干细胞。体外和体内诱导骨髓干细胞在海绵体内成骨。

The use of porous ceramics is desirable as a scaffold for osteogenesis by undifferentiated mesenchymal stem cells. In the scaffold, an active proliferation and differentiation to hard tissue formation cells was expected for the cells. Therefore an effective factor to differentiate the cells was examined. As a result, it was recognized in hyaluronic acid, laminin and leucine to promote the cell proliferation and differentiation effectively. And it was recognized that the dextran was effective for the differentiation of the undifferentiated mesenchymal cells. The ceramics scaffold received the bone marrow stem cells in the pores. However, it was found that the attachment of the bone marrow stem cells in the sponge with fibrous structure was impossible because of the fibrous structure. Coating of the dextran to the sponge gave width to the fiber, and attachment of bone marrow stem cells would be possible. Oteogenesis in the sponge by bone marrow stem cells was induced in vitro and in vivo.