The cryopreservation of umbilical cord blood derived mesenchymal stem cells and the possibility of clinical application of those cells to cleft lip and palate patient.

这项研究是用脐带来源的间充质干细胞进行的，因为脐带血来源的间充质干细胞无法得到确认。冻存3个月后，脐带间充质干细胞的增殖能力和多能性明显低于骨髓间充质干细胞。骨髓间充质干细胞在骨诱导液中易在成骨细胞样细胞中分化，但脐带间充质干细胞的生物成骨活性较低，即使在脐带间充质细胞用b-FGF增殖后再加BMP-2进行骨诱导也是如此。因此，作为再生骨的细胞来源，我们认为深低温保存的脐带或血液来源的间充质干细胞在临床上的应用是困难的。

This research was done with the umbilical cord derived mesenchymal stem cells, because the cord blood derived mesenchymal stem cell couldn't be confirmed. Three months of cryopreservation later, the proliferation potency and multipotency of the umbilical cord derived mesenchymal stem cell were apparently inferior to those of bone marrow derived mesenchymal stem cell. The bone marrow derived mesenchymal stem cell differentiated easily in the osteoblast-like cell under the bone induction medium, but the biological bone viability of the umbilical cord derived mesenchymal stem cell was low, even if bone induction was done with added BMP-2 after the umbilical cord derived mesenchymal cell proliferated with b-FGF. Therefore, as cell source for the regenerated bone, We thought that clinical application of the cryopreserved mesenchymal stem cell from an umbilical cord or blood origin was difficult.